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yvdF [2019-06-18 10:41:20]
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yvdF [2019-06-18 10:41:20]

glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase
locus
BSU34620
pI
5.18
mw
68.68 kDa
protein length
589 aa Sequence Blast
gene length
1770 bp Sequence Blast
function
starch and maltodextrin utilization
product
glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.23|Utilization of starch/ maltodextrin]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,555,902 3,557,671

    The protein

    Protein family

  • [SW|glycosyl hydrolase 13 family] (according to Swiss-Prot)

    • Structure

  • [PDB|1J0J] (from G. stearothermophilus, 56% identity, complex with maltotetraose), [PDB|1J0H] (from G. stearothermophilus, 56% identity)

    • [SW|Localization]

  • on both sides of the cytoplasmic membrane and in the periplasm during vegetative growth but in the cytoplasm of prespores [Pubmed|19465663]

    • Expression and Regulation

    Operons

    genes
    [gene|EA1BEC9FCFD2A32142A8A8E6D7362DB37E02052A|yvdE]-[gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]-[gene|5771E1976D986638B13ADDE97EDE32709E355923|mdxE]-[gene|EA2DD9892C1128ADCD6C8BC61248EBE45339E6D6|mdxF]-[gene|D72CFFE8ED3EC0280AE92C05A136D66ED1F62251|mdxG]-[gene|68C6FD5D4D9491EE15A5015447959A45C36EA702|yvdJ]-[gene|5F615BB886EC4F7000AAD42258DB8E47616552DE|yvdK]-[gene|6E5AB4620F9A896C32CD77AF314C67035814AE0A|malL]-[gene|CAFAE305B1771DD89E81F75A6713161393BE99A0|pgcM]
    description
    [Pubmed|16707683]

    regulation

  • induced in the presence of maltose [Pubmed|9573215]
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    Biological materials

    Mutant

  • MGNA-B628 (yvdF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1627 NBRP B. subtilis, Japan]
  • BKE34620 ([gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT, downstream forward: _UP4_TAACATTCTGTTATCGGTAA
  • BKK34620 ([gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT, downstream forward: _UP4_TAACATTCTGTTATCGGTAA

    • References

  • 16707683,19465663,15650689